Full Article: PDF
Scientific Object Identifier: http://s-o-i.org/1.1/TAS-04-108-26
DOI: https://dx.doi.org/10.15863/TAS.2022.04.108.26
Language: English
Citation: Hamzah, F., & Hamzah, N. (2022). Identification Of The Bright-Greenish-Yellow-Fluorrescence (Bgy-F) Compound On Cotton Lint Associated With Aflatoxin Contamination N Cottonseed. ISJ Theoretical & Applied Science, 04 (108), 133-138. Soi: http://s-o-i.org/1.1/TAS-04-108-26 Doi: https://dx.doi.org/10.15863/TAS.2022.04.108.26 |
Pages: 133-138
Published: 30.04.2022
Abstract: In order to characterize the structure of the bright-yellow-fluorescence (BGY-F) compound on cotton lint associated with aflatoxin contamination in cotton seed, various in vitro and in vivonatural BGY-F reaction products were prepared. Under similar high pressure liquid chromatography separation with variable wavelength and programmable fluorescence detection (HPLC-UV-FL), combined with atmospheric pressure ionization and mass spectral determinations it was found that the BGY-F reaction products prepared from three preparation: (a)kojic acid (KA)+peroxidase (soybean peroxide or horseradish type IV and type II) + H2O2, or (b) detached fresh cotton locules +KA+H2O2 or (c) attached field cotton locules that were treated with a spore suspension of aflatoxigenic Aspergillus flavus, all resulted in identical chromatographic characteristics, and all exhibited a molecular weight of 282. Further characterization of the BGY-F reaction products with 1H-and 13C-NMR spectroscopic analysis revealed that it was a dehydrogenator dimmer of 2 KA, linked through the C-6-positions
Key words: Acid Dimmer, Aflatoxin; Aspergillus flavus; Bright-greenish-yellow fluorescence (BGY-F);gossypium hirsutum l; HPLC-UV/F-MS; kojic acid; Malvaceae; NMR.
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