ISJ Theoretical & Applied Science 04(72) 2019

Philadelphia, USA

Satte, M., Osman, T., Yousif, A., Badawi, K., Zayed, M., & Nourein, I.

Effect of the long-term gum Arabic solutions preservation on histological structure of biological tissues.

Full Article: PDF

Scientific Object Identifier: http://s-o-i.org/1.1/TAS-04-72-4

DOI: https://dx.doi.org/10.15863/TAS.2019.04.72.4

Language: English

Citation: Satte, M., Osman, T., Yousif, A., Badawi, K., Zayed, M., & Nourein, I. (2019). Effect of the long-term gum Arabic solutions preservation on histological structure of biological tissues. ISJ Theoretical & Applied Science, 04 (72), 20-29. Soi: http://s-o-i.org/1.1/TAS-04-72-4 Doi: https://dx.doi.org/10.15863/TAS.2019.04.72.4

Pages: 20-29

Published: 30.04.2019

Abstract: In our previous study by Satte et al., in 2017, gum Arabic solutions were used in the preservation of biological tissues, and the produced samples were maintained their macroscopic features for the long-term. The current study was planned to compare the microscopic structure of biological tissue preserved in gum Arabic solutions with those of unpreserved tissue as a negative control and tissue preserved in silicone-S10 as a positive control. The study was conducted on 5 µm thick tissue sections obtained from 39 specimens of adult sheep, divided into eleven experimental groups and two control groups (positive & negative); each group contained three specimens: kidney, heart, and brain. The experimental groups were preserved in eleven different concentrations of gum Arabic solutions, the positive control group was preserved in silicone-S10, and the negative control group was unpreserved tissue (fresh). From each group, five tissue sections of 5 µm thick were cut from kidney, heart, and brain and were prepared for subsequent hematoxylin and eosin staining for histological examination under a light microscope. The gum Arabic solutions preserved tissue sections revealed the histological architecture similar to that of the unpreserved and that observed in silicone-S10 preserved tissues. The present study concludes that; tissues preserved in gum Arabic solutions can be used in teaching histology for medical students.

Key words: histology, gum Arabic, preservation, biological tissues.