Boymirzaev, A. S., Sotiboldiev, B. S., & Sobirjonova, S. T.
Evaluation of double electrical layer in pores of macroporous glasses in exclusion chromatography. |
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Full Article: PDF
Scientific Object Identifier: http://s-o-i.org/1.1/TAS-06-98-32
DOI: https://dx.doi.org/10.15863/TAS.2021.06.98.32
Language: Russian
Citation: Boymirzaev, A. S., Sotiboldiev, B. S., & Sobirjonova, S. T. (2021). Evaluation of double electrical layer in pores of macroporous glasses in exclusion chromatography. ISJ Theoretical & Applied Science, 06 (98), 287- 291. Soi: http://s-o-i.org/1.1/TAS-06-98-32 Doi: https://dx.doi.org/10.15863/TAS.2021.06.98.32 |
Pages: 287-291
Published: 30.06.2021
Abstract: The article deals with the determination of the thickness of the electric double layer in the pores of macroporous glasses, which are used as a sorbent in size exclusion chromatography of polymers. Inorganic sorbents such as silica gels and macroporous glasses are widely used for the analysis of macromolecules in size exclusion chromatography due to the rigidity of their matrix and large pore volume. The thickness of the electrical layer or the Debye screening radius on the surface of silica sorbents and in the chains of macroions can be determined using the theories of Debye-Huckel, Flory-Fox, and Mark-Kuhn-Hauwink, as well as the principle of universal calibration in size exclusion chromatography at different ionic strengths in water salt solvent.
Key words: electric double layer, macroporous glass, polyacrylic acid, ionic strength, Debye screening radius, universal calibration, electrostatic forces.
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