Berdimbetova, G. E., Boymirzaev, A. S., & Karlibaeva, B. P.
Synthesis and characteristics of carboxymethylchitosan from the Artemia parthenogenetica cyst of the Aral sea. |
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Full Article: PDF
Scientific Object Identifier: http://s-o-i.org/1.1/TAS-12-116-37
DOI: https://dx.doi.org/10.15863/TAS.2022.12.116.37
Language: Russian
Citation: Berdimbetova, G. E., Boymirzaev, A. S., & Karlibaeva, B. P. (2022). Synthesis and characteristics of carboxymethylchitosan from the Artemia parthenogenetica cyst of the Aral sea. ISJ Theoretical & Applied Science, 12 (116), 480-492. Soi: http://s-o-i.org/1.1/TAS-12-116-37 Doi: https://dx.doi.org/10.15863/TAS.2022.12.116.37 |
Pages: 480-492
Published: 30.12.2022
Abstract: The article discusses the conditions for obtaining chitin from substandard cysts of Artemia parthenogenetica of the Aral Sea, according to the traditional scheme of sequential deproteinization, demineralization and decolorization of raw materials, followed by deacetylation of chitin to chitosan and the synthesis of carboxymethylchitosan. When obtaining chitosan based on chitin from Artemia cysts, a harsh deacetylation method was used using a 50% concentrated alkali solution, high temperature conditions (120-1300C) and a long processing time (5-6 hours). Further, carboxymethylchitosan was synthesized from the obtained chitosan. The results of the chemical composition of the feedstock (quality, substandard and crushed cysts), as well as the main qualitative characteristics of chitin and chitosan, including color, average molecular weight, solubility, content of residual mineral substances, etc., are summarized. The structures of chitin/chitosan and carboxymethylchitosan were characterized by spectroscopic methods, the degree of chitosan deacetylation and the degree of substitution of carboxymethylchitosan were calculated. Size exclusion liquid chromatography was used to study the polyelectrolyte properties, molecular weight parameters and polydispersity of chitosan and carboxymethylchitosan. In addition, surface morphology and thermal properties were analyzed by scanning electron microscopy, thermogravimetric analysis, and differential scanning calorimetry, respectively.
Key words: Artemia parthenogenetica, cysts, chitin, chitosan, carboxymethylchitosan, infrared spectroscopy, nuclear magnetic resonance spectroscopy, size exclusion liquid chromatography.
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